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5-Point Guide To Buying A New Microscope For Your Lab
Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss – this can be a frustrating process. A lot of scientists and students consider the...
5 Techniques For Dramatic Improvements In Reproducibility
Written By: Heather Brown-Harding, PhD It's not easy to improve reproducibility in your experiments. Image manipulation has become a major problem in science, whether intentional or accidental. This has exploded with the advent of digital imaging and software like Photoshop....
3 Compensation Mistakes That Will Ruin Your Flow Cytometry Experiments
Written By: Tim Bushnell, PhD Putting it basically, compensation is the mathematical process of correcting spectral spillover from a fluorochrome into a secondary detector. Yet even the most talented rookie researchers may find themselves at a loss when it comes...
7 Individual Artifacts In Fluorescence Microscopy And How To Minimize Them
Written By: Heather-Brown Harding, PhD There are 7 different “artifacts” that may be affecting the quality of your imaging. Before digging into the details, let’s begin by defining an artifact: Essentially, it is any error introduced through sample preparation, the...
We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results
Written By: Tim Bushnell, PhD As a follow-up to our post on tSNE where we compared the speed of calculation in leading software packages, let’s consider the case of SPADE (Spanning-tree Progression Analysis of Density-normalized Events). A favored algorithm in...
Mass Cytometry Revolves Around These 5 Things
Written By: Tim Bushnell, PhD Today’s article will summarize the functionality of mass cytometry technology. This tech has been commercialized largely by Fluidigm in the CyTof systems. There are 5 key points to cover, or takeaways, that cytometrists should keep...
Use These 5 Techniques for Super Resolution
Written By: Heather Brown-Harding, PhD When you need better resolution than what can be achieved using a traditional microscope, it can be very intimidating to figure out which microscopes will work best for your experiment. Super-resolution imaging methods require software...
Understanding Reproducibility in Flow Cytometry – It’s the Antibodies!
Written By: Tim Bushnell, PhD Reproducibility is key to the scientific method. After the results of a study are published, the community validates the findings and extends them. If the findings are not reproducible, the second step is impossible. With...
3 Components Of Every Flow Cytometer You Don’t Know Enough About
Written By: Tim Bushnell, PhD All flow cytometer instruments have a certain 3 components you likely don’t know enough about. The way they are put together will dictate the performance of the system. As a user, you’ll be interacting heavily...
Books
Advanced Microscopy
Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.
Modern Flow Cytometry
Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more.
Advanced 4-10 Color Compensation
Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.