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4 Factors To Improve Flow Cytometry Cell Sorting Speed
Written By: Tim Bushnell, PhD Cell sorting owes a lot to Mack Fuwyler - when he developed the first cell sorter, he started a revolution. Finally, researchers had a tool to isolate specific cells of interest. In the intervening years,...
Optical Tissue Clearing For Pristine Sample Preparation
Optical tissue clearing is an essential step in high-resolution microscopy. Heather-Brown Harding, PhD, discusses the available techniques. Imaging deep into tissues has always been difficult, whether challenges derive from working distance, light absorption by natural chromophores, or light scattering by...
4 Flow Cytometry Assays For Monitoring Intracellular Processes
Written By: Tim Bushnell, PhD The most common flow assay is undoubtedly immunophenotyping, in which fluorescently tagged antibodies are used to bind to cellular proteins. This allows you to determine the types of cells present. As long as there is...
Discover The Myriad Applications Of Beads In Flow Cytometry
What is the single-most important feature of a flow cytometry experiment? Arguably, it’s the stained cells that gather data about biological processes of interest. However, a flow cytometer can measure cell-like particles as well as cells, which opens the realm...
5-Point Guide To Buying A New Microscope For Your Lab
Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss – this can be a frustrating process. A lot of scientists and students consider the...
5 Techniques For Dramatic Improvements In Reproducibility
Written By: Heather Brown-Harding, PhD It's not easy to improve reproducibility in your experiments. Image manipulation has become a major problem in science, whether intentional or accidental. This has exploded with the advent of digital imaging and software like Photoshop....
3 Compensation Mistakes That Will Ruin Your Flow Cytometry Experiments
Written By: Tim Bushnell, PhD Putting it basically, compensation is the mathematical process of correcting spectral spillover from a fluorochrome into a secondary detector. Yet even the most talented rookie researchers may find themselves at a loss when it comes...
7 Individual Artifacts In Fluorescence Microscopy And How To Minimize Them
Written By: Heather-Brown Harding, PhD There are 7 different “artifacts” that may be affecting the quality of your imaging. Before digging into the details, let’s begin by defining an artifact: Essentially, it is any error introduced through sample preparation, the...
We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results
Written By: Tim Bushnell, PhD As a follow-up to our post on tSNE where we compared the speed of calculation in leading software packages, let’s consider the case of SPADE (Spanning-tree Progression Analysis of Density-normalized Events). A favored algorithm in...
Books
Advanced Microscopy
Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.
Modern Flow Cytometry
Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more.
Advanced 4-10 Color Compensation
Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.