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Understanding Reproducibility in Flow Cytometry – It’s the Antibodies!
Written By: Tim Bushnell, PhD Reproducibility is key to the scientific method. After the results of a study are published, the community validates the findings and extends them. If the findings are not reproducible, the second step is impossible. With...
3 Components Of Every Flow Cytometer You Don’t Know Enough About
Written By: Tim Bushnell, PhD All flow cytometer instruments have a certain 3 components you likely don’t know enough about. The way they are put together will dictate the performance of the system. As a user, you’ll be interacting heavily...
4 Ways To Analyze Tissues By Flow Cytometry
Written By: Tim Bushnell, PhD Why are we interested in cytometry? What are its practical applications? Let’s start by defining cytometry as the measurement of cellular processes at the whole-cell level. This definition is useful because it includes not only...
Ask These 7 Questions Before Purchasing A Flow Cytometer
Written By: Tim Bushnell, PhD Using funds to make a capital purchase can be an exciting time in a facility. If you don’t have the funds in hand, planning for future purchases requires due diligence to make sure the investment...
6 Microscopy Assays To Determine Cell Health and Improve Your Experimental Results
Written By: Heather Brown-Harding, PhD When you're performing imaging, we always want to make sure that any phenotype we see isn't just an artifact of unhealthy cells or if you're doing drug discovery, you want to ensure that the treatment...
7 Things You Didn’t Know About Imaging Cytometry
Written By: Tim Bushnell, PhD We are visual creatures. We seek to capture and describe the world around us. Some of the earliest evidence of this is found in the cave paintings that have been found around the world, like...
4 Steps To Implementing a QC Program For Your Flow Cytometry Experiments
Written By: Tim Bushnell, PhD With the emphasis on reproducibility in science, it is important to look at the process of quality control and quality assurance and how these can help improve the reproducibility of data. First, what is the...
5 FlowJo Hacks To Boost The Quality Of Your Flow Cytometry Analysis
Written By: Tim Bushnell, PhD Primary data analysis, that is the analysis at the sample or tube level, is where the populations of interest are identified and the necessary data is extracted for secondary analysis. Since the creation of the...
5 Special Considerations for Live Cell Imaging
Written By: Heather Brown-Harding, PhD Live cell imaging is advantageous for research were you may be worried about artifacts of fixation or when you want to measure a phenomenon over time. Live cell imaging is more difficult to achieve than...
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How to Perform Doublet Discrimination In Flow Cytometry
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Written by Tim Bushnell, PhD What is doublet discrimination? You are probably familiar with the term, “doublet discrimination” or “doublet exclusion”, and have likely included this flow cytometry measurement into at least some (if not all) of your gating strategies....
How To Analyze FACS Data And Prepare Flow Cytometry Figures For Scientific Papers
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Written by Tim Bushnell, PhD “It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.” ― Albert Einstein...
What Is A Fluorescence Minus One, or FMO Control
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Written by Tim Bushnell, PhD The Fluorescence Minus One Control, or FMO control is a type of control used to properly interpret flow cytometry data. It is used to identify and gate cells in the context of data spread due...
How To Set And Monitor Optimal Voltages For A Flow Cytometry Experiment
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Written by Tim Bushnell, PhD When a researcher first sits down in front of the flow cytometer, they are faced with several choices. One of the most confusing is, “What voltage do I set my detector at?” Unless the researcher...
3 Flow Cytometry Gates That Will Improve The Accuracy Of Your FACS Data Analysis
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Written by Tim Bushnell, PhD Flow cytometry data analysis typically involves a step where a series of gates are defined to identify the population of interest. The development of robust gating strategies, and the communication of these strategies can be...
Books
Advanced Microscopy
Expert Books
Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.
Modern Flow Cytometry
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Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more.
Antibody Compensation
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Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.
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