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Discover The Myriad Applications Of Beads In Flow Cytometry

What is the single-most important feature of a flow cytometry experiment? Arguably, it’s the stained cells that gather data about biological processes of interest. However, a flow cytometer can measure cell-like particles as well as cells, which opens the realm...

5-Point Guide To Buying A New Microscope For Your Lab

Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss – this can be a frustrating process. A lot of scientists and students consider the ...

5 Techniques For Dramatic Improvements In Reproducibility

Written By: Heather Brown-Harding, PhD Image manipulation has become a major problem in science, whether intentional or accidental. This has exploded with the advent of digital imaging and software like Photoshop. There are even mobile applications like Instagram filters that...

3 Compensation Mistakes That Will Ruin Your Flow Cytometry Experiments

Written By: Tim Bushnell, PhD Putting it basically, compensation is the mathematical process of correcting spectral spillover from a fluorochrome into a secondary detector. Yet even the most talented rookie researchers may find themselves at a loss when it comes...

7 Individual Artifacts In Fluorescence Microscopy And How To Minimize Them

Written By: Heather-Brown Harding, PhD There are 7 different “artifacts” that may be affecting the quality of your imaging. Before digging into the details, let’s begin by defining an artifact: Essentially, it is any error introduced through sample preparation, the...

We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results

Written By: Tim Bushnell, PhD As a follow-up to our post on tSNE where we compared the speed of calculation in leading software packages, let’s consider the case of SPADE (Spanning-tree Progression Analysis of Density-normalized Events). A favored algorithm in...

Mass Cytometry Revolves Around These 5 Things

Written By: Tim Bushnell, PhD Today’s article will summarize the functionality of mass cytometry technology. This tech has been commercialized largely by Fluidigm in the CyTof systems. There are 5 key points to cover, or takeaways, that cytometrists should keep...

Use These 5 Techniques for Super Resolution

Written By: Heather Brown-Harding, PhD When you need better resolution than what can be achieved using a traditional microscope, it can be very intimidating to figure out which microscopes will work best for your experiment. Super-resolution imaging methods require software...

Understanding Reproducibility in Flow Cytometry – It’s the Antibodies!

Written By: Tim Bushnell, PhD Reproducibility is key to the scientific method. After the results of a study are published, the community validates the findings and extends them. If the findings are not reproducible, the second step is impossible. With...

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How to Perform Doublet Discrimination In Flow Cytometry

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Written by Tim Bushnell, PhD What is doublet discrimination? You are probably familiar with the term, “doublet discrimination” or “doublet exclusion”, and have likely included this flow cytometry measurement into at least some (if not all) of your gating strategies....

How To Analyze FACS Data And Prepare Flow Cytometry Figures For Scientific Papers

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Written by Tim Bushnell, PhD “It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.” ― Albert Einstein...

What Is A Fluorescence Minus One, or FMO Control

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Written by Tim Bushnell, PhD The Fluorescence Minus One Control, or FMO control is a type of control used to properly interpret flow cytometry data.  It is used to identify and gate cells in the context of data spread due...

3 Flow Cytometry Gates That Will Improve The Accuracy Of Your FACS Data Analysis

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Written by Tim Bushnell, PhD Flow cytometry data analysis typically involves a step where a series of gates are defined to identify the population of interest. The development of robust gating strategies, and the communication of these strategies can be...

When To Use (And Not Use) Flow Cytometry Isotype Controls

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Written by Tim Bushnell, PhD Antibodies can bind to cells in a specific manner - where the FAB portion of the antibody binds to a high-affinity specific target or the FC portion of the antibody binds to the FcR on...

Books

Advanced Microscopy

Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.

Modern Flow Cytometry

Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more. 

Antibody Compensation

Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.

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