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My Proven 5-Point Fast Track To A Career In Flow

  Written By:Tim Bushnell, Ph.D. While we normally cover purely technical content for application in your lab, this week’s article is a bit different. I want to talk about a highly effective career option that I know from many years...

Up Your Stain Game With These 7 Non-Fluorescent Histology Dyes

Written By: Heather Brown-Harding, PhD Histological stains that have an affinity for specific cellular components have been in use since at least the 1770s when John Hill used carmine to study tissues. The variety of stains exploded during the 1800s...

3 Ways Flow Cytometry Can Be Used To Research Bacteria

  Written By: Tim Bushnell, PhD The global bacterial biomass has been estimated to be 5x1030, which is significantly higher than plants and animals. We are intimately dependent on bacteria for processing waste, producing vitamin B12, fixing nitrogen and so...

Avoid Flow Cytometry Faux Pas: How To Set Voltage The Right Way

Written By: Tim Bushnell, PhD The Shift from Analog To Digital Cytometer Age Analog instruments processed data differently than the current generation of digital instruments. With analog systems, if the populations were “off-scale,” especially at the low end of the...

Designing Microscopy Experiments Related To Infectious Diseases And Antivirals

Written By: Heather Brown-Harding, PhD The novel coronavirus (SARS-CoV-2) emerged in Wuhan, China, in December 2019  and spread across the globe becoming the pandemic that the world is reeling with today. Currently, COVID-19  has no targeted therapies approved by the...

Getting A New Flow Cytometer? Try Before You Buy (And 2 Other Tips)

Written By: Tim Bushnell, PhD One question that I get asked on a regular basis is what flow cytometer should I purchase?  It’s not as simple as you might imagine. In fact, you need to treat this process as carefully...

My 3-Step Panel Validation Pocket Guide

Written By: Tim Bushnell, PhD Experimental validation refers to the process of evaluating a panel and ensuring it’s robust. Sometimes, you're just handed a panel that you have to follow, and it might not be the best panel for your...

Easy-To-Forget Flow Fundamentals That Thwart Bad Science

Written By: Tim Bushnell, PhD No matter how advanced or cutting-edge your study is, good science is profoundly dependent on the fundamentals. In fact, no matter your experience level, it is always good to revisit the fundamentals now and again....

Important Controls For Your Flow Cytometry Lab

Written By: Tim Bushnell, PhD No researcher wants to discover that the results of a long, careful experiment are confounded by an uncontrolled variable. To assist in data interpretation, you must build careful controls into your experimental workflow. These controls...

Books

Advanced Microscopy

Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.

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flow cytometry tablet eBook cover

Modern Flow Cytometry

Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more. 

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Advanced 4-10 Color Compensation

Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.

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