6 – Microscopy

5 Essential Controls For Reproducible Fluorescent Microscopy Imaging

By: Heather Brown-Harding, PhD

Controls are an integral part of all science. And the complexity of fluorescent microscopy makes including the right controls in your experiments paramount. You should be including these 5 controls in your experiments: an unlabeled sample, a non-specific binding control, a positive and negative control, an antibody titration curve, and blinded image capture. With those controls, you can be sure that your experiments are what you think they are and perform your imaging with confidence. So, happy imaging!

Microscopy - 5 Reasons Coverslips Are Important For High-Quality Imaging

By: Heather Brown-Harding, PhD

Most people are familiar with coverslips being placed on slides to protect the sample, but that's not the only reasons that coverslips are important. They also affect the image quality. Coverslips function by working with your microscope to focus light to a single point and avoiding unnecessary noise in your image. Having the wrong type of coverslip will damage the quality of your images and the quality of the data you extract from those images.

5 Keys To Writing A Shared Instrument Grant

By: Tim Bushnell, PhD

I’m a scientist, not Perry Mason. I’m not a defense attorney or a detective. Why would I say that? Because well before March grant writers need to be a Perry Mason – make the case and defend it to have a successful Shared Instrument Grant (SIG). For those running core facilities (or Shared Resource Laboratories), then March is a critical time of the year, and we’re not talking basketball. A grant is a true story. This year’s deadline is March 21. It’s critically important in this tight funding climate to make sure nothing moves your grant from the “must-fund” to…