Skip to content

What Is Dynamic Range

Written by Tim Bushnell, PhD

Dynamic range is the total range of fluorescent values obtained from a particular flow cytometry assay. It is defined as the ratio of the largest possible fluorescent signal to the smallest possible fluorescent signal.

The dynamic range can vary based on the application. For example, a cell cycle assay may have a dynamic range of only 1000 fluorescence units. Surface staining against CD3 may have a dynamic range of 10,000.

There is some debate as to the largest dynamic range required for flow cytometry, with some estimates putting the largest required dynamic range at about 3.5 and others arguing for a larger dynamic range. This has lead to the proliferation of instruments with expanded scales to allow for 6, 7, and even up to 10 units of dynamic range.

Tim Bushnell, PhD

BOOKS

Advanced Microscopy

Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.
flow cytometry tablet eBook cover

Modern Flow Cytometry

Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more. 

Advanced 4-10 Color Compensation

Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.

Top 40 Networking Scripts For PhDs

If you want to get replies from top employers and recruiters, this ebook is for you. These networking scripts will show you the exact words ...

Informational Interviews For PhDs

If you want to learn how to set up and execute informational interviews with PhDs working in industry, this ebook is for you. Here, you ...

Industry Resume Guide For PhDs

If you have a PhD and want to create the perfect industry resume to attract employers, this ebook is for you. Here, you will get ...

Top 20 Industry Jobs For PhDs

If you want to learn about the top 20 industry careers for PhDs regardless of your PhD background, this ebook is for you. Here, you ...

Salary Negotiation For PhDs

If you have a PhD and want to learn advanced salary negotiation strategies, this ebook is for you. Here, you will learn how to set ...

Top 20 Transferable Skills For PhDs

If you want to learn the top 20 transferable skills the industry employers ranked as most important for PhDs to include on their resumes and ...

Related Posts You Might Like

flow cytometer being tested

Getting A New Flow Cytometer? Try Before You Buy (And 2 Other Tips)

Written By: Tim Bushnell, PhD One question that I get asked on a regular basis is what flow cytometer should I purchase?  It’s not as ...
Read More

5-Point Guide To Buying A New Microscope For Your Lab

Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss ...
Read More

Ask These 7 Questions Before Purchasing A Flow Cytometer

Written By: Tim Bushnell, PhD Using funds to make a capital purchase can be an exciting time in a facility. If you don’t have the ...
Read More

Instrument Quality Control For Reproducible Flow Cytometry Experiments

Written by Tim Bushnell, PhD The NIH has released a series of reproducibility guidelines that scientists must address. These guidelines have been introduced because there's ...
Read More

How to Optimize Flow Cytometry Hardware For Rare Event Analysis

Written by Tim Bushnell, PhD “Not everything that can be counted counts and not everything that counts can be counted.” — William Bruce Cameron (but ...
Read More

3 Ways The ZE5 Cell Analyzer Accelerates Flow Cytometry Research Opportunities

Written by Tim Bushnell, PhD As new instruments come on the market, vendors are quick to provide data proving the systems’ prowess including sensitivity, speed, ...
Read More