Master The Field of Flow Cytometry

Welcome To Expert Cytometry (ExCyte) Mastery Class

The Expert cytometry training program is for scientists who want to:

  • Stay up to date with current trends and best practices
  • Gain advanced knowledge quickly (even if you’re a beginner)
  • Learn how to control for any type of flow cytometry experiment
  • Learn specific flow cytometry techniques and methodologies
  • Get expert information on flow cytometry antibody panel design, experimental design, data analysis, and statistics

Join The Waitlist

When Expert Cytometry opens up to new members, you'll be the first to know. You will also be notified about promotions and free webinars for Expert Cytometry.

 

Enter Your Info Below To Get On The Waitlist for Expert Cytometry

Join The Waitlist

When Expert Cytometry opens up to new members, you'll be the first to know. You will also be notified about promotions and free webinars for Expert Cytometry.

 

Success Stories

Fabienne Lucas, MD, PhD

Fabienne Lucas, MD, PhD

Brigham and Women's Hospital
Resident in Clinical Pathology

I began Expert Cytometry as a postdoc in the US after my medical and PhD training in Germany and the UK. I had used flow cytometry for more than 5 years but needed to take a ‘deep dive’ to really understand the technique. I soon became the expert in my lab and helped my peers design their experiments. It was also the impetus for me to apply (and receive) the Mary Lou Ingram Scholarship, and start the MiSET RFC standard while I continue my career in cytometry.

Carina Torres

Carina Torres

Core Manager
Eli Lilly & Company

I invited ExCyte to Lilly Pharmaceuticals to teach a two-day comprehensive course; ranging from the fundamentals of flow to the experimental design of assays specific to what I think a lot of biotech companies are doing these days. Afterward, several of our scientists wondered why I didn’t bring in a course like this earlier! Our instructor, Tim Bushnell, Ph.D., educated my users with the background they needed to design their assays most effectively. I immediately noticed the positive impact he had when my users began to ask more informed questions during the planning stages of their experiments. As a busy core manager, it’s difficult to find the time to prepare such a well-designed course like ExCyte offers while trying to stay focused on innovation and maintaining a competitive advantage in the fast-paced industry environment. I’m grateful to the ExCyte team for helping me to keep things moving forward at Lilly.

Amrutesh Puranik, PhD

Amrutesh Puranik, PhD

Postdoctoral Fellow
Mayo Clinic

I’m a postdoctoral researcher and know a lot about flow but I needed to learn a few new techniques and execute them before I could get my paper published. I joined Expert Cytometry because they have a good reputation for teaching high-level flow cytometry information very well. I really enjoyed the personal guidance the Cheeky Scientist Technical Program offered and was impressed by how much I learned about controls and compensation, experimental design, statistics, and even apoptosis. Cheeky Scientist's ExCyte is perhaps the only class that teaches you from scratch how to be a flow cytometry expert.

Miguel Garcia

Miguel Garcia

Core Manager
Swiss Federal Institute of Technology

As a Core Manager, my position requires staying up to date with the latest best practices. The Cheeky Scientist Technical Programs provided the necessary information on flow cytometry to stay on the cutting edge of the field for a reasonable investment. Overall, the course was really well done by speakers expert on the field and I really appreciated the flexibility of listening to the webinars as needed. The Cheeky Scientist Technical Program is wonderful and I would recommend it to beginner as well as advanced flow cytometry professionals.

About Expert Cytometry

A PhD-level flow cytometry training program with 8 modules, each including a one-hour instructional video along with a downloadable Workbooks and Presentations.

After the completion of each module, you can test your expertise against quizzes and scored exams. Topics include Advanced Statistics, Advanced Experimental Design, Advanced Proliferation, Advanced Compensation, Advanced Data Analysis, Advanced Controls, Advanced Antibody Panel Design, Advanced Apoptosis, Advanced FCS Express, Advanced FACSDiva, Image Stream Analysis, Cell Sorting, CyTOF and Higher Order Analysis, Clinical Flow Cytometry, Microparticle Detection and Analysis, and much more.

Here's What You Get Immediate Access To After Becoming An Mastery Class Member

Module #1: Instrumentation

This module covers the process of generating data within a flow cytometer by covering the three major components of every instrument: fluidics, optics, and electronics.

We will explain how a particle goes from the “sample” to data file within the flow cytometer. The aim is to clarify how the three components of the cytometer are intertwined and work together to create the complex, rich data files that flow cytometry was developed to generate.

Module #2: Reagents

This module introduces users to the toolkit of specific probes most commonly used in flow cytometry to characterize the form and function of cellular and sub-cellular components and specimens.

We define fluorescence and describe common fluorescence processes that result in a measurable light signal that corresponds to biological phenotype or activity. Hundreds of natural and man-made fluorescent compounds are available to explore and measure biological phenomena. Different types of specific probes are introduced, including fluorescently tagged antibodies, transgenic fluorescent proteins, nucleic acid binding probes, fluorescent enzyme substrates, and cell division tracking dyes.

The merits of different fluorophores are compared in terms of brightness and usefulness in multiplex labeling strategies. Multicolor staining combinations maximize the amount of information gleaned from a single sample, but introduce special challenges due to overlap in the color of light emitted by many commonly used fluorophores. To separate and identify fluorescent signals from multiple probes we apply a mathematical correction to compensate for the natural crosstalk between many dyes.

Module #3: Controls

The aim of this module is to guide you, the user, regarding the possible sources of variation that may be encountered when preparing, collecting, and analyzing flow cytometry data.

Once the sources of variation are covered, techniques are presented to minimize variation and to yield high precision quantitation. Many of the techniques involve the use of appropriate bead standards for standardizing the instrument, compensation, flow rate, and event rate. In addition, tips are suggested regarding staining controls and the use of reference biological material. Lastly, data analysis strategies are presented that can be employed for objective region drawing.

Module #4: Data Analysis

In this module, you, the flow cytometry user, will receive an introduction to the basic parameters of flow cytometry data analysis.

Starting with the generation of the data file itself contained in the Flow Cytometry Standard, .FCS, we will discuss the various ways of presenting the raw data as graphical plots, how to draw regions and create gates, and the basic statistics used in many applications.

Lastly, we briefly touch on the subject of comparing data sets; that is, a group of control samples and a group of experimental samples, and how these data are generated.

Enter Your Info Below To Get On The Waitlist for Expert Cytometry

Join The Waitlist

When Expert Cytometry opens up to new members, you'll be the first to know. You will also be notified about promotions and free webinars for Expert Cytometry.

 

Success Stories

Fabienne Lucas, MD, PhD

Fabienne Lucas, MD, PhD

Brigham and Women's Hospital
Resident in Clinical Pathology

I began Expert Cytometry as a postdoc in the US after my medical and PhD training in Germany and the UK. I had used flow cytometry for more than 5 years but needed to take a ‘deep dive’ to really understand the technique. I soon became the expert in my lab and helped my peers design their experiments. It was also the impetus for me to apply (and receive) the Mary Lou Ingram Scholarship, and start the MiSET RFC standard while I continue my career in cytometry.

Carina Torres

Carina Torres

Core Manager
Eli Lilly & Company

I invited ExCyte to Lilly Pharmaceuticals to teach a two-day comprehensive course; ranging from the fundamentals of flow to the experimental design of assays specific to what I think a lot of biotech companies are doing these days. Afterward, several of our scientists wondered why I didn’t bring in a course like this earlier! Our instructor, Tim Bushnell, Ph.D., educated my users with the background they needed to design their assays most effectively. I immediately noticed the positive impact he had when my users began to ask more informed questions during the planning stages of their experiments. As a busy core manager, it’s difficult to find the time to prepare such a well-designed course like ExCyte offers while trying to stay focused on innovation and maintaining a competitive advantage in the fast-paced industry environment. I’m grateful to the ExCyte team for helping me to keep things moving forward at Lilly.

Amrutesh Puranik, PhD

Amrutesh Puranik, PhD

Postdoctoral Fellow
Mayo Clinic

I’m a postdoctoral researcher and know a lot about flow but I needed to learn a few new techniques and execute them before I could get my paper published. I joined Expert Cytometry because they have a good reputation for teaching high-level flow cytometry information very well. I really enjoyed the personal guidance the Cheeky Scientist Technical Program offered and was impressed by how much I learned about controls and compensation, experimental design, statistics, and even apoptosis. Cheeky Scientist's ExCyte is perhaps the only class that teaches you from scratch how to be a flow cytometry expert.

Miguel Garcia

Miguel Garcia

Core Manager
Swiss Federal Institute of Technology

As a Core Manager, my position requires staying up to date with the latest best practices. The Cheeky Scientist Technical Programs provided the necessary information on flow cytometry to stay on the cutting edge of the field for a reasonable investment. Overall, the course was really well done by speakers expert on the field and I really appreciated the flexibility of listening to the webinars as needed. The Cheeky Scientist Technical Program is wonderful and I would recommend it to beginner as well as advanced flow cytometry professionals.

4 total core training, each including several instructional videos along with a downloadable workbooks and presentations.
100+ training videos to dig into topics like apoptosis, panel design, fluorescent proteins, experimental design, proliferation, and statistics.
10+ total training quizzes to keep you challenged between the core modules and the Advanced Video Training series.
Private access to the online group to connect and collaborate with other passionate learners.
Access to at least one webinar per month about current topics in flow cytometry
Network with other passionate scientists to help you keep your lab performance on the cutting edge.

Enter Your Info Below To Get On The Waitlist for Expert Cytometry

Join The Waitlist

When Expert Cytometry opens up to new members, you'll be the first to know. You will also be notified about promotions and free webinars for Expert Cytometry.