Another very common laser after the “blue” and “red” laser in flow cytometers. A “violet” laser in flow cytometry typically is referred to as the 405 because most flow cytometers use a violet laser with a wavelength of 405nm. Pacific Blue and Pacific Orange are the most common fluorophores used with this laser, but Brilliant Violet fluors are gaining popularity.
ABOUT TIM BUSHNELL, PHD
Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.More Written by Tim Bushnell, PhD