What Is A Sample Injection Port
In flow cytometry, cells, in suspension are moved from the tube to the interrogation point and finally into the waste (or to be sorted, but that is a different story). To do this, the fluidics components of the flow cytometry are required.
The fluidics are comprised of a running (or Sheath) fluid, that runs through the system in laminar flow. The movement of this sheath can be achieved by several mechanisms, the most common method using pressure provided by pumps.
The second component of the fluidics is the sample injection port (SIP). This is where the sample is pushed through to be introduced to the sheath fluid. Based on the principles of hydrodynamic focusing, these cells are become strung out, single file, in the direction of the flow, where they will pass the interrogation point.
The final main component of the fluidics is the flow cell, which is where everything comes together.
ABOUT TIM BUSHNELL, PHD
Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.More Written by Tim Bushnell, PhD