The second most common laser in a flow cytometer after the “blue” laser. The “red” laser typically has a wavelength of 633nm, but new flow cytometers are starting to use a “red” laser with a wavelength of 640nm. The most common fluorophores excited and detected off this laser are APC, Alexa Fluor 660, Alexa Fluor 700, and APC-tandems.
ABOUT TIM BUSHNELL, PHD
Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.More Written by Tim Bushnell, PhD