Skip to content

Phosphate Buffer

Written by Tim Bushnell, PhD

PBS is the acronym for phosphate buffered saline. Phosphate buffer is one of the most common buffers used in biological research.  The phosphate serves as a buffer to keep the pH constant, while the saline is referencing the osmolarity.  Additional ions such as Ca2+ or Mg2+ , energy sources like glucose, or chelators such as EDTA can be added based on the specific needs of the experiment.

There are many different formulations of PBS, based on the cell type and needs.  The most common, from Cold Spring Harbor is shown below, providing a pH of 7.4 with an osmolarity to normal human cells.

Phosphate Buffer

 

Tim Bushnell, PhD

BOOKS

Advanced Microscopy

Learn the best practices and advanced techniques across the diverse fields of microscopy, including instrumentation, experimental setup, image analysis, figure preparation, and more.
flow cytometry tablet eBook cover

Modern Flow Cytometry

Learn the best practices of flow cytometry experimentation, data analysis, figure preparation, antibody panel design, instrumentation and more. 

Advanced 4-10 Color Compensation

Advanced 4-10 Color Compensation, Learn strategies for designing advanced antibody compensation panels and how to use your compensation matrix to analyze your experimental data.

Top 40 Networking Scripts For PhDs

If you want to get replies from top employers and recruiters, this ebook is for you. These networking scripts will show you the exact words ...

Informational Interviews For PhDs

If you want to learn how to set up and execute informational interviews with PhDs working in industry, this ebook is for you. Here, you ...

Industry Resume Guide For PhDs

If you have a PhD and want to create the perfect industry resume to attract employers, this ebook is for you. Here, you will get ...

Top 20 Industry Jobs For PhDs

If you want to learn about the top 20 industry careers for PhDs regardless of your PhD background, this ebook is for you. Here, you ...

Salary Negotiation For PhDs

If you have a PhD and want to learn advanced salary negotiation strategies, this ebook is for you. Here, you will learn how to set ...

Top 20 Transferable Skills For PhDs

If you want to learn the top 20 transferable skills the industry employers ranked as most important for PhDs to include on their resumes and ...

Related Posts You Might Like

5-Point Guide To Buying A New Microscope For Your Lab

Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss ...
Read More

Ask These 7 Questions Before Purchasing A Flow Cytometer

Written By: Tim Bushnell, PhD Using funds to make a capital purchase can be an exciting time in a facility. If you don’t have the ...
Read More

Instrument Quality Control For Reproducible Flow Cytometry Experiments

Written by Tim Bushnell, PhD The NIH has released a series of reproducibility guidelines that scientists must address. These guidelines have been introduced because there's ...
Read More

How to Optimize Flow Cytometry Hardware For Rare Event Analysis

Written by Tim Bushnell, PhD “Not everything that can be counted counts and not everything that counts can be counted.” — William Bruce Cameron (but ...
Read More

3 Ways The ZE5 Cell Analyzer Accelerates Flow Cytometry Research Opportunities

Written by Tim Bushnell, PhD As new instruments come on the market, vendors are quick to provide data proving the systems’ prowess including sensitivity, speed, ...
Read More

3 Advantages Of Using The ZE5 Cell Analyzer

Written by Tim Bushnell, PhD Since the first laser was mounted to create the first flow cytometer, there has been a push for more - ...
Read More