Counted by the Photomultiplier Tube (PMT) in the flow cytometer. Photons enter the PMT and the signal is amplified in the PMT when a photon strikes the anode and “knocks” of electrons. These electrons then hit a series of subsequent anodes, amplifying the total number of electrons of signal. The PMT then counts the total number of electrons and this is converted to the signal.
Latest posts by Tim Bushnell, PhD (see all)
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