Counted by the Photomultiplier Tube (PMT) in the flow cytometer. Photons enter the PMT and the signal is amplified in the PMT when a photon strikes the anode and “knocks” of electrons. These electrons then hit a series of subsequent anodes, amplifying the total number of electrons of signal. The PMT then counts the total number of electrons and this is converted to the signal.
ABOUT TIM BUSHNELL, PHD
Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.More Written by Tim Bushnell, PhD