The most common laser type in a flow cytometer. Typically, this laser has a wavelength of 488nm in flow cytometers. In fact, the term “Blue” laser is often interchanged with “488” laser. Frequently used fluorophores excited and detected by this laser are FITC, Alexa Fluor 488, PE, PerCP, and their tandems.
ABOUT TIM BUSHNELL, PHD
Tim Bushnell holds a PhD in Biology from the Rensselaer Polytechnic Institute. He is a co-founder of—and didactic mind behind—ExCyte, the world’s leading flow cytometry training company, which organization boasts a veritable library of in-the-lab resources on sequencing, microscopy, and related topics in the life sciences.More Written by Tim Bushnell, PhD