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Logicle Scaling

An implementation of biexponential scaling published by the Herzenberg lab at Stanford. The biexonential scale is a combination of linear and log scaling on a single axis using an arcsine function as its backbone. The “logicle” implementation of biexponential was implemented in many popular software packages like FACSDiva and FlowJo. Other types of biexponential scaling exist, including Hyperlog. Biexponential scales are more generally referred to as hybrid scales and include other variations like lin/log or log with negative.

More information on logicle sclaing can be found here:

Parks DR, Roederer M, Moore WA. (2006). A new “Logicle” display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. Cytometry. 69: 541-545

Tim Bushnell, PhD

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