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While controls are critical for minimizing the effects of variables in your flow cytometry experiments, choosing the right controls are essential. When your research is published, reviewers need to see that your variables have been analyzed properly. Evaluating strengths and weaknesses will give you information and back up arguments for the case for or against isotype controls. Here’s a review of what isotype controls are and if you need to use them.

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From photon, through conversion from light, into an electron, into an electronic signal pulse. The journey of a photon, through to its data representation via flow cytometry, involves the detection system and the electronics, and basically following a bouncing photon to its ultimate digitization. This article provides 3 key takeaways in the process from photons through to end data for analysis.

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