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Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here. Understanding how the fluidics system works on your flow cytometer will help you prevent many common issues, prepare your samples correctly, and protect your data. Here are four important things to consider about the fluidics system in a flow cytometer.

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Fluorescence compensation is one of the more difficult, understandably confusing, and misunderstood aspects of flow cytometry. Understanding what compensation is, why it is necessary, and what to expect when using it, are critical for generating useful and high-quality data from flow cytometry experiments. The definition and mechanics of flow cytometry compensation and the critical concept that compensation’s most basic principle relies on are discussed here.

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