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Good flow cytometry depends on a high quality, single cell suspension. If the cells put through the instrument are not of high quality, the ensuing data will be difficult to analyze. Likewise, if the sample is clumpy, one will not be able to readily distinguish cells of interest from the clumps they are attached to. Sample preparation becomes the critical first step in any flow cytometry experiment. To get high quality results, follow these 3 sample preparation steps.

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This article is the second in a two-part series outlining some of the major components of the optical systems used in flow cytometry to provide insight and understanding to what happens before a signal is produced from the PMT detectors. Serving as a knowledge toolkit that can help troubleshoot problems you may encounter when performing your next cytometry experiment, this article investigates lenses, mirrors and filters in your flow cytometry equipment.

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Understanding the optical system of a flow cytometer may seem unnecessary for performing a typical experiment, but the more you know about your instrument, the better you will be at understanding your data, as well as troubleshooting potential issues. This article breaks down 4 elements of flow cytometer optics to provide a broad understanding on its impact on fluorescence.

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