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The last 40 years have seen significant advancements in cell sorting technology. Cell sorting is often the entry point for many experiments. Fluorescent Activated Cell Sorting (FACS) combines the traditional power of flow cytometry and couples it with the ability to isolate the cells of interest. Understanding the inner workings of the instruments and some rules for preparing samples will lead to more successful experiments. Here are 4 essential facts about FACS.

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Multicolor sorting experiments can be complicated and if not setup properly, result in wasted time and suboptimal results. When setting up a multicolor experiment, the most saliently critical step is to set PMT voltages properly. In addition, using a viability dye and addressing doublet discrimination and setting the right sort regions and gates is important for any kind of flow cytometry experiment, but particularly for cell sorting. These tips help to ensure your setup is perfect to achieve results of the highest caliber.

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