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When preparing figures for publication, the scientific question and hypothesis that forms the basis of the paper must be central and all the figures must be in support of that. The flow cytometry data that forms the basis of the conclusions should be presented clearly and concisely. While it provides pretty pictures and colorful layouts, the meat of the data are the numbers ― percentages of populations, fluorescent intensity levels and the like ― are what will convince the reader that the hypothesis tested is valid and well thought out. Here’s how to choose the correct flow figure for presenting your data.

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Cell sorters have become more sophisticated to rival the multicolor capabilities of analytical cytometers with cell sorting experiments becoming more complicated to match. While multicolor sorts are very feasible and can yield excellent results, success is always a product of very careful planning and optimization. From choosing optimal fluorophores to strategies to reducing spillover spreading, this comprehensive article is broken into two parts to give you all you need to play your experiments for advanced accuracy.

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