Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated.
Unfortunately, as Begley and Ellis pointed out in a commentary in Nature that when Amgen attempted to reproduce 53 “landmark” papers in the area of cancer research, only 6 papers were “confirmed.” What does that mean to you, the flow cytometry researcher? To avoid publishing errors that reviews despise, it’s important to follow and promote the best practices in the field, thus ensuring that your data is reproducible to investigators attempting to validate your research. In particular, you must follow these 5 experimental tips.Read More
T regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens.
Tregs decrease inflammation via the secretion of immunosuppressive cytokines (IL-10, TGF-b) and also through direct suppression of inflammatory effector T cells (such as Th1 and Th17 cells). Given the importance of this unique T cell subset in so many immune responses, many investigators feel remiss if they immunophenotype their cell populations of interest without including a Treg measurement in the mix. But quantifying Tregs can be complicated. This article will show you how to quantify Tregs and how to ensure you’re measuring true suppressor T cells.Read More