The cell sorting process is inherently stressful.
Cells are first manipulated in suspension for up to several hours to prepare and stain them. Then, during the cell sorting process, these cells are pushed through narrow tubing under high pressure in the range of approximately 10-70 PSI, rapidly depressurized after passing through a nozzle, and then jetted through the air at velocities of 20 m/s (~44 MPH) or higher. Keeping cells healthy, happy, vital, and viable over the course of a cell sorting experiment is important to keep cells alive during the sort but also that the recovery of cells from the sort is high. Here are three things you can do to help ensure high levels of viability.Read More
The Complete Blood Count is a powerful addition to many flow cytometry workflows.
The CBC is an automated hematology test that looks at the levels of all the cells in your blood, providing your physician with valuable information about your health. Using just a small sample of blood, the CBC generates an extensive amount of information WITHOUT the need for centrifugation or multi-color staining experiments. Running a CBC is fast, easy, and inexpensive. In the world of clinical research, a CBC should always be run on the human clinical research samples. As a result, any obvious outliers can be removed from the study, reducing the spread of the data and reducing the risk of confounding your interpretation of the data. Here are the major advantages of obtaining a CBC by flow cytometry.Read More