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Electrostatic cell sorting is a complicated process that continues to be improved. It can be a struggle to understand exactly how all of the sorting components coalesce to accomplish the cell sorter’s tasks. For many scientists, the most difficult parts of the sorting equation are how droplets are charged and how drop delays are calculated. By understanding these two things, you will be in a better position to set up a successful fcell sorting experiment, which will help you achieve high sort recovery values, allowing for the accurate analysis of your cells and more cells to work with for your downstream experiments. Here’s how cell sorting droplets are charged and how cell sorting drop delays are determined.

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Flow Cytometry is a remarkably powerful tool for the study of T cells. It has been successfully used for many decades to accurately visualize and enumerate a variety of T cell subsets. With a large sensitivity range for fluorescent probes, >95% sampling efficiency, and the ability to sort populations of interest for further study, fluorescent-based cytometry remains a tool of choice for T cell analysis. The key is to define your T cell populations of interest with correct gating strategies and to back up your T cell subset findings with functional analysis of these subsets. A cell’s actions should guide its definition, not the other way around.

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The International Cytometry Certification Exam was developed over a period of several years. The goal was to ensure a base level of flow cytometry knowledge in certificate holders. Some cytometrists have deemed the ICCE as unnecessary. Others have voiced concerns about the specialization of certain exam subsections. However, despite these concerns, the ICCE is here to stay. The exam and certification process as a whole has the support of multiple companies that are providing training, as well as the support of ISAC, ICCS, and the Wallace H. Coulter Foundation. In the end, the most telling test of the value of the ICCE will be when flow cyotmetry job postings in basic research start asking for the certification. This day may not be too far away.

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