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All the experiments and experience in the world do not count if you are unable to communicate your results to the scientific community. As part of that communication process, your paper will undergo the dreaded ‘Peer-Review’ process. If you wish your paper to survive this process, you must collect, analyze, and present your flow cytometry data properly—before you submit your paper. A review of the following questions, as well as how to answer them, will help ensure your paper is not rejected. Here are 5 specific questions reviewers will ask when reviewing your flow cytometry data.

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To make certain your instrument is set up correctly for your experiments, manufacturers have developed defined polystyrene beads.

These beads’ consistent nature helps you to assess how your instrument is behaving, helps you set up proper compensation matrices, and helps you generate volumetric counts of your cell populations. Alignment, sensitivity, and fluidic quality control beads will help you to ensure that with the same wattage on the laser and the same voltage applied to the detector returns the same median fluorescence. The right compensation capture beads will bind antibodies of multiple isotypes from multiple species and give you a very bright positive signal from which you can calculate a correct compensation matrix. The use of counting beads allows you to easily calculate your cell concentration in your sample. Together, these beads will make your life easier and help you get your data published. Here are the 3 beads you should use.

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