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The field of flow cytometry is moving beyond the use of isotype controls, with many suggesting they be left out of nearly all experiments.

Yet, isotype controls were once considered the only negative controls you should ever use. They are still very often included by some labs, almost abandoned by others, and a subject of confusion for many beginners. What are they, why and when do I need them? Are they of any use at all, or just a waste of money? Most importantly, why do reviewers keep asking for them when they review papers containing flow data? Here is everything you need to know about using (or not using) isotype controls in your next flow cytometry experiment.

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With the increased development of fluorescently conjugated monoclonal antibodies came more applications with potential clinical impact.

In bone marrow transplantation, studies using hematopoietic cytokines made it feasible to gather stem cells from peripheral blood. It was also shown that reconstitution of bone marrow was accelerated when using cell from peripheral blood rather than bone marrow. Many more clinical flow cytoemtry applications have been developed. All of which should follow these 6 keys of running clinical flow cytometry experiments.

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