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Written by Tim Bushnell, PhD We all know that flow cytometry makes individual measurements on large populations of cells, it allows for statistical analysis of the data, lending strength to a researcher’s conclusions. Likewise, the isolation of very complex populations by flow cytometry cell sorting can help lead to a richer understanding of the intricate…

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Written by Tim Bushnell, PhD Pairing highly expressed antigens (like CD3) with dimmer fluorochromes, and the antigens of interest with the brightest fluorochromes, is a key part of panel design with few tools to help. With early generation instruments, this was relatively easy to determine, since fluorochrome choice was limited. With the advent of instruments…

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