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Written by Tim Bushnell, PhD Flow cytometry is a powerful tool for asking and answering questions at the whole cell level. The first step in any flow cytometry experiment is to define the hypothesis or biological question that is to be answered.  This helps ensure that flow is the correct technique for answering the question.…

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Written by Tim Bushnell, PhD Flow cytometry can be an intimidating tool for the new cytometrist.  There are many sources that one can turn to for training and education. Local Shared Resource Lab Manager If you institute has a shared resource lab (a ‘core facility’), look in what training they provide.  This is your first…

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Written by Tim Bushnell, PhD Cell sorting remains the best tool to isolate and purify cellular populations that can be phenotypically defined. This is especially true for rare-event detection and purification. Successful rare event detection and purification requires some attention to ensure the best yield and purity. 1) Watch the instrument to ensure success a)…

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Written by Tim Bushnell, PhD Mass Cytometry, commercialized by the company DVS Sciences, in the instrument called the CyTOF is a newly emerging technology in the field of flow cytometry.  This technology replaces traditional fluorescent-labeled antibodies with highly purified, stable isotopes with very well characterized mass values.  This extends the power of flow cytometry from…

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  Written by Tim Bushnell, PhD Cell sorting can be a scary proposition. A precious sample is introduced into a machine that pressurizes the cells to 70 PSI, moves them past one or more lasers, vibrates the stream at 90 kHz before decelerating the cells to atmospheric pressure before they hit an aqueous surface. Many…

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