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Written by Tim Bushnell, PhD Cell Sorting is the process of isolating cells after the identification of the cells using the principles of flow cytometry. The upstream components of the cell sorter are common to all flow cytometers. The difference comes in what is done with the cells after they have been interrogated and identified.…

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A variation on biexponential scaling similar to logicle scaling. The biexonential scale is a combination of linear and log scaling on a single axis using an arcsine function as its backbone. Biexponential scales are more generally referred to as hybrid scales and include other variations like lin/log or log with negative. More information on Hyperlog…

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Written by Tim Bushnell, PhD Cell cycle analysis by flow cytometry uses a DNA binding dye, such as propidium iodide (PI), 7- aminoactinomycin D (7-AAD) or 4’,6-diamidino-2phenylindole (DAPI), to determine the cell cycle state of a cell population. The Gap1 (G1) phase of an eukaryotic cell is defined as having 2C DNA. The synthesis (or…

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Written by Tim Bushnell, PhD The question always arises as to what is the top cell sorter on the market. This question is a difficult one to generalize because there are several considerations that need to be made in choosing a cell sorter. What are the sorting needs of the investigators? If all the investigators…

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