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Another very common laser after the “blue” and “red” laser in flow cytometers. A “violet” laser in flow cytometry typically is referred to as the 405 because most flow cytometers use a violet laser with a wavelength of 405nm. Pacific Blue and Pacific Orange are the most common fluorophores used with this laser, but Brilliant…

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The second most common laser in a flow cytometer after the “blue” laser. The “red” laser typically has a wavelength of 633nm, but new flow cytometers are starting to use a “red” laser with a wavelength of 640nm. The most common fluorophores excited and detected off this laser are APC, Alexa Fluor 660, Alexa Fluor…

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The most common laser type in a flow cytometer. Typically, this laser has a wavelength of 488nm in flow cytometers. ┬áIn fact, the term “Blue” laser is often interchanged with “488” laser. Frequently used fluorophores excited and detected by this laser are FITC, Alexa Fluor 488, PE, PerCP, and their tandems.

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A filter that allows light between a set wavelength to pass through and reflects light above and below the set wavelength. For example, a bandpass filter with a wavelength of 550/40nm would allow light between 530nm and 570nm to pass through, but reflect light below 530nm and above 570nm.

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A filter that allows light over a set wavelength to pass through and reflects light above the set wavelength. For example, a shortpass filter with a wavelength of 450nm would allow light with a wavelength less than 450nm to pass through the filter, but reflect light higher than 450nm.

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